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A study on leptospiral infection among cows and pigs by culture and microagglutination test
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Joung Soon Kim, Hee Jung Kim, Yong Heo, Sun Il Park, Byung Chun Lee, Hyun Chil Mun, Sung Sam Kim, Young Hee Oh
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Korean J Epidemiol. 1989;11(2):191-197.
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 Abstract
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Abstract
This study of leptospiral infections among cows and pigs were carried out to find out possible role of these domestic animals as an infection source for humans, and the magnitude of economic loss due to abortions caused by leptospiral infections among these domestic animals.
Blood samples of 459 cows in May and 446 heads in November 1989 were collected from 19 villages of Kyunggi Province. These samples were cultured and serologically tested.
Kidney of 200 pigs from all over the country were collected from a governmental slaughter house and cultured for isolation of leptospires.
Results obtained are as followings:
1. Only one strain of leptospires, most strongly reactive to Pomona antibody, was isolated from duplicated cultures of 905 cow blood samples in EMJH medium.
2. Serological test by MAT with seven reference strains and three locally isolated strains was positive in 12% of the sample in May and 26% in November samples; positive rate increased slightly as the age of cows increased, however, there was no difference in positive rate by sex, type of cow and area.
3. There was no statistically significant association between abortion history among cows(one year-period) and positive MAT.
4. No leptospires was isolated from 200 pig kidneys cultured, probably because they were all too young to be infected; most of them were around six-month old and raised in professional pig farms.
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Summary
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